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. 2018 Jun 20;8:9411. doi: 10.1038/s41598-018-27779-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Separation performance of our co-flow system. (a) Focusing and separation of fluorescent particles (green) using a 2× dilution blood (red): (i) overlapped pseudocolored images indicating a high-efficiency separation; (ii) the corresponding intensity profiles across the dashed lines in part (i); (iii) micrographs showing the separation outcomes. (b) Quantitative results of separation performance using whole blood and 2× dilution blood spiked with particles and Hep G2 cells. Blood cells instead of RBCs were used here since WBCs were also present in the sample. However, the percentage of WBCs was less than 1%. Error bars represent standard deviation of three tests. (c) WBC and hepatocarcinoma cell (HCC) extracted and identified from clinical sample (2× dilution) in our system. Error bars are standard deviations of three tests. All flow rates were 225 µL/min with α = 1.