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. 2018 Jun 20;8:9401. doi: 10.1038/s41598-018-27760-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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BNC characterisation. (a) BNC biomaterial is formed as fleeces during static culture at the air-liquid interface (I). BNC fleece patch used for cell seeding (II). (III) SEM image (magnification M = 3,000x)²² providing insight into the nanocellulose network ultra-structure. Images I and III were provided by D. Kralisch. (b) Multiphoton pattern of dried BNC. The pattern of rolled BNC rehydrated with PBS dramatically changes within minutes. (c) Simultaneous AF/SHG images from within the BNC exhibit both dot- to elliptic-shaped AF signals and fibre-shaped SHG signals with different signal strengths (MSI, mean signal intensity). Representative overlay image shown in the middle. Irregular structures within the cellulose network were observed as well.