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. 2018 Jun 20;8:9417. doi: 10.1038/s41598-018-27752-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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(a) Variations in amide I Raman bands of the control (down) and S. aureus infected bones (above) up to 21 days. For comparison, spectra were normalized to the CH₂ wag band at 1450 cm⁻¹. (b) Variations in the Amide I band intensity at 1656 cm⁻¹ for the control and infected bones with respect to the number of incubation days. Inset shows the optical images (size: 500 μm × 500 μm) of control and infected bone surfaces showing no considerable visual changes in the samples during the incubation periods.