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. 2018 Jun 20;8:9229. doi: 10.1038/s41598-018-27271-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Introduction of enhanced green fluorescence protein (EGFP)-expressing retrovirus into primary cells of the loggerhead sea turtle. Left panels display EGFP detection of retrovirus-infected cells. Middle panels display cell morphology of retrovirus-infected cells, viewed under a differential interference contrast (DIC) microscope. Right panels display merged images of DIC and fluorescence images. Results with infection at 37 °C (A), 30 °C (B) and 26 °C (C).