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. 2018 Jun 20;8:9229. doi: 10.1038/s41598-018-27271-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Establishment of Cyclin-dependent kinase 4 (CDK4)-, Cyclin D- and telomerase-expressing cells derived from the loggerhead sea turtle. (A) left panel, cell morphology of the loggerhead-derived cells, expressing CDK4, Cyclin D, and telomerase. Right panel, cell morphology of the loggerhead primary cells. (B) Protein expression of the introduced CDK4 and Cyclin D. The expression of human CDK4, human Cyclin D, and endogenous tubulin were detected by using western blot analysis. Lane 1, primary A634 cell line; Lane 2, A634-K4DT cell line; Lane 3, primary A640 cell line; Lane 4, A640-K4DT cell line.