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. 2018 Jun 20;8:9420. doi: 10.1038/s41598-018-27330-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The vibration-induced exercise paradigm did not trigger mitochondrial adaptations in C. elegans. (A) No significant difference was observed for mitochondrial DNA copy number between the exercised and control animals (n ≥ 20 animals per group). Similar trend was observed in two other trials. (B) Representative images of mitochondrial morphology in sarcomeres of young (D7) and old (D12) exercised and control animals, using transgenic strain SJ4103[myo-3p::GFP(mit)] (n ≥ 10 animals per group). Similar trend was observed in two other trials. (C) Oxygen consumption rate (OCR) profiles of the exercised and control animals measured by Seahorse analyser. AUC1: Area under curve (AUC) of the OCR-time graph of the first six time points; AUC2: AUC of the 7^th to 12^th time point; AUC3: AUC of the 13^th to 18^th time point. (D) No significant differences were observed for Basal Respiration (BR), Maximal Respiration (MR) or Spare Respiratory Capacity (SRC) between the exercised and control animals (n = 6 repeats per group; each repeat contains 10 animals). BR = AUC3 – AUC1; MR = AUC2 – AUC3; SRC = AUC2 – AUC1. Experiments in this panel were done one day post-training on day 7 of age.