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. 2018 Jun 20;8:9403. doi: 10.1038/s41598-018-27539-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Gene expression of the three candidate genes in SHRSPrch1.31. Kidneys were collected from the three rat strains at 12–14 weeks of age without salt-loading. Total RNA was extracted and quantitative RT-PCR was performed as previously described (ref.²⁸, see also Methods in the main text). Pr1.31; SHRSPrch1.31, *P < 0.05 vs. SHR, **P < 0.05 vs. SHRSP by Student’s t-test. Five rats were used for each strain.