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. 2018 Jun 20;8:9388. doi: 10.1038/s41598-018-27266-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Average expression levels of serine-glycine synthesis enzymes in cancer cell lines. (A) Schematic representation of pathways of serine-glycine synthesis and one-carbon metabolism. The enzymes examined in this study are highlighted with pink boxes, while cellular compartments are highlighted with green boxes. (B) Immunoblots of serine-glycine synthesis pathway enzymes in the indicated fourteen human cancer cell lines derived from seven human tissues are shown. Βeta-actin was used as loading control. The grouping of immunoblots were obtained from cropped blots obtained from different gels and are delineated by white space between them.