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. 2018 Jun 20;8:9388. doi: 10.1038/s41598-018-27266-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PHGDH and Ki-67 expression in 3D microtumors. (A) Spatial localization of Ki-67 (green), PHGDH (red), and their overlap in mt600 microtumors at low- (top panel), intermediate (middle panel) and high magnification (bottom panel). Confocal images are centered on the middle plane of the microtumor. In the overlay panels Hoechst counterstain (blue color) indicates cell nuclei. PHGDH expression is restricted to the outer layer of Ki-67⁺ cells. Concentric circles in the top right panel depict the outer layer (O), inner layer (I), and core (C) regions of the microtumor. (B) Average of nine radial linescans for Ki-67 (green) and PHGDH (red) fluorescence intensity. (C) Average of Ki-67 and PHGDH fluorescence intensity in each of the microtumor compartments.