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. 2018 Jun 20;8:9412. doi: 10.1038/s41598-018-27728-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cytotoxicity and ATP depletion after treatment with CaEP or ECT with bleomycin. (a–d) Relative survival of different normal and tumour cell lines 72 hours after treatment with increasing concentrations of calcium or bleomycin and in combination with electroporation (AM ± s.e.m.; n = 16 wells in 3 independent experiments). (e–h) The relative concentration of intracellular ATP content 1 hour after treatment with increasing concentrations of calcium or bleomycin and in combination with electroporation (AM ± s.e.m.; n = 4 wells in 3 independent experiments). *p < 0.05, statistical comparisons were performed by one-way ANOVA analysis and compared to control groups by Holm-Sidak testing. All data are normalized to the untreated or electroporated-only cells.