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. 2018 Jun 20;8:9412. doi: 10.1038/s41598-018-27728-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Cell morphology and type of cell death after CaEP or ECT with bleomycin (BLM). (a–d) Black arrows represent necrotic cells with ruptured membranes and cell lysis (example magnified on upper right). Green arrows indicate cells with early signs of apoptosis, such as vacuolisation of cytosol (example magnified on lower right). Morphological changes were assessed in at least two independent experiments with two parallels. (e–h) Percentage of viable cells (negative; green), apoptotic cells (Annexin V positive; purple), late apoptotic/necrotic cells (Annexin V + 7-AAD positive; blue) and necrotic cells (7-AAD positive; red) after different treatments is shown. Each graph corresponds to the cell line in the same row. Bars represent the average of the experiment with two parallels repeated twice.