Table 1.
Examples of digital polymerase chain reaction platforms available
| BioMark HD/EP1 (Fluidigm) | QuantStudio 3D (Life Technologies) | Constellation/Constellation modules (Formulatrix) | Clarity (JN Medsys) | Naica (Stilla Technologies) | RainDrop (RainDance Technologies) | QX200 (Bio-Rad) | |
|---|---|---|---|---|---|---|---|
| Partitions | 765 or 770 | 20,000 | 8000 or 32,000 | 10,000 | 30,000 | 5 × 106–10 × 106 | 20,000 |
| Total reaction volume (μL) | 4–8 | 14.5 | 10 | 15 | 20 | 25–50 | 20 |
| Samples per run | 12 or 48 | 24 | 96 or 24 | 32 | 12 | 8 | 96 |
| Duration (h) | ~4 | ~3 | ~1.5 | ~4 | ~2 | ~7–8 | ~6 |
| Dyes | FAM/EvaGreen, VIC, ROX (Cy5) | FAM/SYBR, VIC, ROX | 5 (FAM/EvaGreen, HEX, ROX, NED, TED, Cy5) for Constellation or 8 different wavelenghts for Constellation Module | FAM/SYBR/EvaGreen, VIC/HEX | FAM, Cy3/VIC/HEX, Cy5 | FAM, VIC | FAM/EvaGreen, VIC/HEX |
| Master mix | Open | Proprietary | Open | Open | Proprietary | Open | Proprietary |