Table 3.
Absolute copy numbers for stock DNA for MON810 and hmgA target determined by three independent laboratories on the same DNA sample by duplex droplet digital polymerase chain reaction and calculated genetically modified (GM) content
Target | Laboratory 1 | Laboratory 2 | Laboratory 3 | Coefficient of variation (%) |
---|---|---|---|---|
MON810 | 2768 | 2775 | 2951 | 9 |
2282 | 2409 | 2599 | ||
hmgA | 76,874 | 71,213 | 80,616 | 11 |
58,566 | 62,665 | 76,312 | ||
GM content (%) | 3.73 ± 0.21 | 3.87 ± 0.25 | 3.54 ± 0.22 | 4.5 |
The results for two dilutions, each tested in duplicate, are presented for each target. The results for GM content are presented as an average from all replicates together with the 95% confidence interval