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. 2018 Apr 5;27(5):556–568. doi: 10.1007/s10646-018-1927-3

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 5 — Daphnia magna F1 generations were exposed prenatally to 5-azacytidine and the exposures continued for an additional 1 day or 5 days after the release of neonates from the brood chamber of the mother. The exposed F1 generations were then transferred to clean media and maintained for an additional 7 days (recovery period). Control samples were generated following the same procedure without the addition of 5-azacytidine. a Percentage of methylation level for CpG sites located in five different genes (LIMCH1, HCFC1, cdk, G-protein, GALT) as measured via direct bisulfite sequencing. Numbers on the x-axis represents CpG sites while y-axis represents percentage of methylation level. b Heat map demonstrating averaged methylation level for each gene. * Significantly different from respective control (p < 0.05, t-test, n = 3 ± SD)