Figure 10.

Mean endothelial tube length following treatment with conditioned media harvested from normoxia, intermittent and sustained hypoxia treated EC-CFUs with and without ROS inhibitors. (A) Tube formation by EAhy926 endothelial cells grown for 24 hrs on ECM-Gel with conditioned media harvested from normoxia (Norm), intermittent hypoxia (IH) and sustained hypoxia (SH) treated EC-CFUs (n = 3). Inhibitors used: 1 mM N-acetylcysteine (NAC), 100 μM apocynin and 5 μM diphenyl iodide (DPI). For a positive control, EAhy926 endothelial cells were grown with EGM-2 medium supplemented 20% FCS, and for a negative control, DMEM medium supplemented with 20% FCS was used (p < 0.017 for IH and SH vs. Norm, ^*p < 0.05, ^**p < 0.01 compared to Norm in each condition group), as specified in Materials and Methods (B) Representative photomicrographs (X10) of endothelial tube formation with EGM-2 without and with 1 mM NAC, and with DMEM. (C) Representative photomicrographs (X10) of endothelial tube formation with Norm, IH and SH conditioned media using the specified inhibitors.