Figure 11.

Whole well images of endothelial tube formation by EAhy926 endothelial cells following treatment with EC-CFUs conditioned media from normoxia, intermittent and sustained hypoxia using various inhibitors. (A) Whole well tube formation by EAhy926 endothelial cells grown for 24 hrs on ECM-Gel with EGM-2 medium without and with NAC, and with DMEM medium (as specified in Figure 10). (B) Whole well tube formation by EAhy926 endothelial cells grown for 24 hrs on ECM-Gel with conditioned media harvested from normoxia (Norm), intermittent hypoxia (IH) and sustained hypoxia (SH) treated EC-CFUs. Inhibitors included 1 mM N-acetylcysteine (NAC), 100 μM apocynin and 5 μM diphenyl iodide (DPI). In (A,B), High-resolution whole-well imaging at x10 magnification with post-acquisition automatic image stitch were performed.