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. 2018 Jun 14;9:447. doi: 10.3389/fneur.2018.00447

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Copyright © 2018 Avezov, Aizenbud and Lavie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Protein carbonyl formation in EC-CFUs cultures exposed to normoxia, intermittent and sustained hypoxia. Analysis of protein carbonyls levels was based upon 2,4-dinitrophenylhydrazine (DNPH) carbonyl derivation, following immunodetection by using Western Blot (WB) assay with anti-dinitrophenol (DNP) antibodies, followed by densitometric quantitation. (A) Average densitometric quantitation of WB assays of EC-CFUs cultures from 4 different donors exposed to normoxia (Norm), intermittent hypoxia (IH) and sustained hypoxia (SH). (p < 0.017 for: Norm vs. IH) (B) A representative WB analysis of total intracellular protein carbonyls from EC-CFUs cultures in Norm, IH, and SH. (C) The control corresponding Ponceau S total protein stain of the membrane.