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. 2018 Jun 14;9:447. doi: 10.3389/fneur.2018.00447

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Copyright © 2018 Avezov, Aizenbud and Lavie.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The effects of normoxia, intermittent and sustained hypoxia on the expression of NADPH oxidase p22phox subunit in EC-CFUs. p22phox specific fluorescence intensity (FI) was detected using confocal microscopy in EC-CFUs cultured under normoxia (Norm), intermittent hypoxia (IH) and sustained hypoxia (SH). Fixed cells were stained with mouse anti-p22phox primary Abs (diluted 1/100) followed by 1/400 CF 647 anti-mouse staining (red). Nuclei were stained with DAPI (blue), in 3 independent experiments. (A) Average FI of p22phox subunit specific expression per colony. (B) Average FI of specific p22phox subunit expression in EC-CFUs per culture well. (C) Representative photomicrographs of p22phox subunit expression in each of the EC-CFUs treated under Norm, IH and SH (X40).