Figure 5.

Murine peritoneal macrophages response ectromelia virus (ECTV) infection was dependent on Tlr9 and cGas–Sting–Irf3 pathway. Peritoneal macrophages generated from wild-type (WT), cGas^−/−, Sting^gt/gt, Tlr9^−/−, and Irf3^−/− mice were non-infected or infected with ECTV at an MOI of 5. Supernatants were collected 18 h post-infection (hpi) for determining the concentrations of IFN-β by using enzyme-linked immunosorbent assay (ELISA) (A,B), and cells were collected at 15 and 18 hpi for western blot analysis using anti-phospho-Tbk1, anti-Tbk1, anti-phospho-Irf3, and anti-Irf3 (C,D). β-Actin was used as a loading control. The ELISA data were averaged from two independent experiments in biological triplicate. Results of western blot analysis shown are representative of two independent experiments. The data were analyzed using a t-test on SPSS software. In this figure, ND, not detected; **P ≤ 0.01 and ***P ≤ 0.001.