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. 2018 Jun 14;9:1240. doi: 10.3389/fmicb.2018.01240

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Copyright © 2018 Arellano, Martinell García, Rodríguez Plaza, Lara Ortiz, Schreiber, Volkmer, Klipp and Del Rio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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IP-1 induces cell death in MATα cells arrested chemically in G₁/G₀ phase. BY4742 (MATα) cells in the exponential phase were synchronized with nocodazole in M phase, then arrested with TBT in G₁/G₀ phase, and treated with 10 μM IP-1 or IP-1-CO-NH₂. Cell cycle analysis and the FUN 1 assay were performed after each treatment. Treatment with nocodazole and TBT is represented by an asterisk (^∗) for abbreviation. (A) Dot plot, cell aspect ratio vs. cell death fluorescence intensity, allowing for identification of cells in G₁/G₀ phase (red box), cells in S phase (orange box), or cells in G₂/M phase (blue box); the number in each box refers to the percentage of each cell phase in 15,000 cells analyzed. (B) FUN1 cell viability assay. A bivariate plot of the intensity in red and green channels was generated from the in-focus population. Red area contains live cells while green area contains dead cells. The percentage of live cells among the 15,000 cells analyzed in each condition is indicated in the corresponding color. Only one experimental result is presented in this image, but these results were confirmed by a second independent experiment not shown.