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. 2018 Jun 14;9:1240. doi: 10.3389/fmicb.2018.01240

FIGURE 8.

FIGURE 8

IP-1-CO-NH2 induces cell death only when cells are arrested in their cell cycle. The CDC28-as1 strain was used for cell cycle arrest with 10 μM NM-PPI CDC28 permeable inhibitor; staining cells with FUN1 was done in order to quantify the dead cells using an Amnis® microscope-cell-cytometry station (see section “Materials and Methods”). Each panel presents the areas where live (green) and dead (red) cells are located by plotting the fluorescence of living cells (green fluorescence) against the dead cell fluorescence (red fluorescence). (A) CDC28-as1 viability control. (B) CDC28-as1 in the presence of IP-1 which is used as a control for the expected number of dead cells. (C) CDC28-as1 in the presence of CDC28p inhibitor NM-PPI. (D) CDC28-as1 in the presence of CDC28p inhibitor NM-PPI and IP-1-CO-NH2. Each panel presents the results of 15,000 events. Only one experimental result is shown in this image, but these results were confirmed by a second independent experiment not shown.