Table 1.

Yeast strains used in this study.

Strain	Genotype	Source
BY4741	HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0	Open Biosystems, yeast parental strain collection, glycerol stocks
BY4742	HIS3Δ1 LEU2Δ0 LYS2Δ0 URA3Δ0	Open Biosystems, yeast parental strain collection, glycerol stocks
BY4741 null mutants	The following genes were replaced with the KanMX4 marker: ATG11, AYT1, BRE2, CMS1, COX17, CYC3, DEP1, DNM1, DRS2, ELM1, ERV46, FRT2, FUN14, GAT3, ISA2, MHT1, NTG1, NUP60, OYE2, PEP4, POR1, PSR2, SAW1, SEO1, SIR4, SNC1, SNF7, SPO7, SPO75, STE20, STE4, SWC3, SWD1, SYN8, UBR2, UIP3, UTH1, ATG3, ATG19, TOR1, MEH1, SLM4, GTR2, VAC8, NVJ1, PEP4, AUP1, UTH1, GEA1, IRS4, VTC1, VTC2, VTC3, VTC4, VPS51, VPS52, VPS53, VPS54, YPT6, PBS1, PHO81, RIM15, FIG1, CCH1, MID1, LRG1, RVS161, PEA1, PEA2, STE5, STE7, STE11, STE50, FUS3, KSS1, BEM1, BNI1, KAR4, RME1, HAL9, XBP1, HMS1, SKN7, PUT3, MSN4, MGA1, FKH2	Open Biosystems, YKO MATa strain collection, glycerol stocks
ACLY394	W303 (MATa): BAR1Δ CDC28-as1 ΔPRM1::PPRM1-YFP::HIS5+	Shokat et al., 2000; glycerol stock
ACLY387	W303 (MATa): BAR1Δ ΔPRM1::PPRM1-YFP::HIS5+	Shokat et al., 2000; glycerol stock
BY4741 ρ0	HIS3Δ1 LEU2Δ0 MET15Δ0 URA3Δ0	This study; glycerol stock