Table 3.

Oligonucleotides used in this study to verify the proper insertion of the KanMX4 cassette to generate null mutants.

Oligonucleotide	Sequence (5′ → 3′)
KanC Fwd	TGATTTTGATGACGAGCGTAAT
NatC Fwd	TACCAACAAATACAAGCCTACA
Ste11D Rvs	ATCTTACTTGATTTTATTCCAGGGG
Ste50D Rvs	CATTATCCAAACATGAAAATAAGGC
Ste7D Rvs	TGGTTGTGGCATAAAAATAAAGAAT
Ste5D Rvs	GAATGAAAAGCAATATACGCAAGAT
Fus3D Rvs	AATCACTACTTTGGTAGTTTGACGC
Kss1D Rvs	GTGTTGATATCGCCTCTTTGATTAC
Far1D Rvs	TGCTACAACCATGTTGGTATAATTG
Fig1D Rvs	AAATTTCTGGAGCTTTGTTACATTG
Bem1D Rvs	CATGCATTATGATTGAGTGGAAATA
Tec1D Rvs	GATGTGTATTGGCTGGTTTACTTCT
Flo1D Rvs	CCAATACTACCGGTACTTGTTCTTG
Phd1D Rvs	ATGTTTCAAAAAGGCATCATATTGT

Oligonucleotide sequences used to verify the proper insertion of the corresponding antibiotic resistance cassette in the deletion mutant strains. KanC Fwd oligonucleotide was the forward primer used to verify the presence of the KanMX4 cassette while NatC Fwd oligonucleotide was used for the NatMX4 cassette. These were used in conjunction with a reverse primer (Rvs) homologous to a region downstream of the deleted gene.