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Intracellular routing of DC-SIGN and its ligand. Monocyte-derived DCs were pulsed with AF405-labeled AZN-D1 for 30 min on ice and transferred to 37°C. Cells were fixed at indicated time points and stained with the CSRD antibody to localize DC-SIGN. Time-course of the co-localization scores of AF405-labeled AZN-D1 (mean ± SEM) and CSRD (DC-SIGN) with (A) EEA1, (B) LAMP1, (C) HLA-DM, and (D) Rab11 (n > 5,000).
