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. 2018 Jun 14;9:1252. doi: 10.3389/fmicb.2018.01252

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Copyright © 2018 Stranghoener, Schippers, Dultz and Behrens.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Scanning electron micrographs of biotic colonization experiments with synthetic basaltic glasses. (A) BAS1 [no residual stress; low Fe(II) content] with clustered cells scattered on the surface. (B) BAS2 [residual stress; low Fe(II) content] showing intense colonization and biofilm formation on the surface. Note also the network of cracks that became altered during the experiment. (C) Enlargement of the area indicated in image (A). Two cell types were observed: cells comparable in shape and size to those observed in incubation experiments and large (up to 10 μm), round shaped cells forming colonies (white arrow). Filamentous structures (nm sized) seem to facilitate cell-surface attachment (black arrow). (D) Enlargement of the area indicated in image (B). A second type of filamentous structures (μm sized) was observed connecting the colonies (white arrow).