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. 2018 Jun 14;11:196. doi: 10.3389/fnmol.2018.00196

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Copyright © 2018 Yu, Jánosi, Liewald, Wabnig and Gottschalk.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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ERP-1 is expressed in cholinergic and GABAergic motor neurons. Fluorescent micrographs, assembled from smaller images, of each one representative animal expressing GFP from the erp-1 promoter (top right) or channelrhodopsin-2 (ChR2)::YFP (i.e., associated with membranes) from the unc-17 promoter (bottom left; specific for cholinergic neurons). Tentative assignment of the ventral nerve cord neuronal cell bodies (starting from the midbody region, i.e., the vulva) shows that ERP-1 is expressed in cholinergic (red lines and neuron names) and GABAergic (green lines and names) neurons, while punc-17 is restricted to the cholinergic neurons only. See Supplementary Figure S2 for a close-up of the vulva region.