Figure 3.

Mutants lacking ERP-1 have mild cholinergic phenotypes. (A) Aldicarb assay, showing fraction of non-paralyzed animals after the indicated time on 1.5 mM aldicarb. Wild-type animals were compared to erp-1(ok462), unc-57(e406) and erp-1; unc-57 double mutants. unc-10/RIM mutants are used as positive controls. For each assay, three experiments with 15–20 animals from different populations were pooled. Shown are means and SEM, statistically significant differences were analyzed for each time point by t-test (*p < 0.05). (B) Swimming cycles per minute were counted for wild-type and erp-1 mutant animals (from three different populations, on three different days) and averaged. Top: scheme of the experiment. A first swimming assay was followed by 90 s of blue light stimulation, a 90 s resting period in the dark, and a second swimming assay. Animals of the indicated genotype (n = 28–30 each), all expressing ChR2 in cholinergic neurons (transgene zxIs6), were either raised in the absence or presence of the essential ChR2 co-factor all-trans retinal (ATR). Shown are means and SEM. ***p < 0.001, *p < 0.05, after paired or unpaired t-test, with Bonferroni correction.