Figure 4.

Disruption of cAMP-dependent sAHP inhibition in PL-mPFC persistently impairs cocaine memory retrieval and subsequent reinstatement. (A) Schematic showing design of behavioral experiments. Below, the CPP tests are shown in (B), and reinstatement is shown in (C). (B) Line graphs revealing that rats given PL-mPFC microinfusions (red arrows) of control (left) or both the cAMP-dependent signaling inhibitor and sAHP inhibitor (right) before the first CPP test only expressed a cocaine CPP across all tests, whereas rats treated with the cAMP inhibitor alone (middle) did not express a cocaine CPP. (C) Line graphs showing that rats given PL-mPFC microinfusions of control (left) or the cAMP inhibitor and sAHP inhibitor (right) before the first CPP test only (shown in B) expressed cocaine-induced reinstatement following extinction, whereas rats treated with the inhibitor of cAMP-dependent signaling alone (middle) did not express cocaine-induced reinstatement. Line graphs represent the mean ± SEM. cAMP⁽⁻⁾, inhibitor of cAMP-dependent signaling (Rp-2’-O-MB-cAMPs); sAHP⁽⁻⁾, slow afterhyperpolarization inhibitor (UCL-2077); *p < 0.05, **p < 0.01, and ***p < 0.001 vs. the saline-paired chamber.