Figure 3.

Diverse Vβ-Jβ rearrangements of α-GalCer/CD1d-reactive T cells. Fragment length analysis profiles of Vβ7-Jβ products are depicted for tetramer-binding cells from the liver. The intensity of fluorescence is represented in arbitrary units as a function of CDR3 length in amino acids. The results from this analysis are representative of the polyclonality of all Vβ-Jβ rearrangements in tetramer-reactive cells observed in the liver, spleen, and thymus for Vβ8.1, Vβ8.2, Vβ7, and Vβ2. Two independent experiments gave similar results.