Table 2.
Sequence analysis of tetramer+ cells utilizing a Vβ7Jβ1.2 rearrangement in individual C57BL/6 mice
| Organ | CDR3β nucleotide sequences
|
Consensus§ | Clone size | ||||
|---|---|---|---|---|---|---|---|
| Total sequences | Unique* | Redundant† | MLE‡ | ||||
| Thymus | Mouse 1 | 97 | 66 | 31 (14) | 241 | SLGGANSDY | 5 |
| Mouse 2 | 164 | 85 | 79 (33) | 233 | SLGGANSDY | 7 | |
| Liver | Mouse 1 | 85 | 73 | 12 (5) | 481 | SLGGANSDY | 4 |
| Mouse 2 | 154 | 66 | 88 (31) | 152 | SLGGANSDY | 4 | |
| Spleen | Mouse 1 | 144 | 64 | 80 (29) | 151 | SLGGANSDY | 8 |
| Mouse 2 | 208 | 103 | 105 (39) | 253 | SLGGGNSDY | 7 | |
*
Number of unique sequences identified once in the organ.
†
Number of sequences identified more than once in the organ. Number in parentheses indicates the number of unique sequences within this redundant subset.
‡
MLE (maximum likelihood estimate) of the number of distinct sequences, calculated with a 95% confidence interval.
§
Sequence of 9-aa CDR3 that is generated by selecting the most highly represented (>20%) amino acid for each position of the CDR3.