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. 2018 Jun 20;3(3):e00204-18. doi: 10.1128/mSphere.00204-18

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Copyright © 2018 Smith et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 3 — Submitochondrial localization of the small TbTim proteins. Proteinase K protection assays of mitochondria (left panels) and mitoplasts (right panels) isolated from T. brucei cells expressing (A) TbTim9-Myc, (B) TbTim10-Myc, or (C) TbTim8/13-Myc were performed. Mitochondria and mitoplasts were treated with various concentrations (0 to 200 µg/ml) of PK as described in Materials and Methods. Proteins were analyzed by SDS-PAGE and immunoblot analysis using anti-myc, anti-Cyt c, anti-TbTim17, anti-mtHsp70, and anti-tubulin antibodies.