FIG 8 .

Effect of small TbTim knockdown on the TbTim17 complexes in mitochondria. (A and B) Mitochondria isolated from wild-type and small TbTim RNAi cells were solubilized with digitonin, and protein complexes were analyzed by blue native PAGE followed by Western blotting with (A) anti-TbTim17 and (B) anti-CytC1 antibodies. (C and D) Two-dimensional BN/SDS-PAGE (2D-PAGE) analysis of mitochondrial extracts (100 µg) collected from wild-type, TbTim9 RNAi (TbTim9 KD), TbTim10 RNAi (TbTim10 KD), and TbTim8/13 RNAi (TbTim8/13 KD) cells grown in the presence of doxycycline (1.0 µg/ml) for 96 h. Blots were probed with (C) anti-TbTim17 and (D) anti-CytC1 antibodies. (E) Densitometric analysis of the TbTim17 signal intensities from blue native PAGE blots and 2D-PAGE blots normalized to their respective CytC1 signals.