Figure 6.

Attenuated induction in Hsp-70 protein synthesis and enhanced constitutive Hsc-70 protein expression in undifferentiated and differentiated cells

(A) Western blot results for Hsp-70 and Hsc-70 during proliferation and differentiation in response to heat shock at 42°C for 3 h and recovered cells following mild hypothermia (33°C) treatment for 6 h; (B) Immunoblotting of Hsc-70 protein expression to identify the fold expression of Hsc-70 in control, heat shocked and post-hypothermia treated cells; (C) RT-qPCR analysis for mRNA expression of Hsp-70 transcripts (Hspa-4, Hspa-9 and Hspa-14) during heat and post-mild hypothermia treatment in undifferentiated and differentiated cells