Figure 3.

HMGA2 is a target of miR‐541 in SCLC cell lines. (A, C) The HMGA2 mRNA expression levels were significantly inhibited by miR‐541 overexpression in SK‐MES‐1 and H226 cells (n = 3 for each cell group). (B, D) The HMGA2 protein levels were significantly downregulated in the miR‐541‐overexpressing SK‐MES‐1 and H226 cells. Lamin B₂ served as the internal control. A bigger field scan of the blot is shown in Fig. S1. (E, F) The immunocytochemistry results revealed that HMGA2 expression levels were decreased in the nucleus of miR‐541‐overexpressing SK‐MES‐1 and H226 cells.