Figure 4.

HMGA2 expression is upregulated in BEAS‐2B induced by miR‐541 inhibitor, and HMGA2 is a direct target of miR‐541. (A) Successful miR‐541 inhibition in BEAS‐2B cells induced by miR‐541 inhibitor. Cells transfected with negative control inhibitor served as the control cells (n = 3 for each cell group). (B) The HMGA2 mRNA expression levels were significantly enhanced by miR‐541 inhibition in BEAS‐2B cells (n = 3 for each cell group). (C) The HMGA2 protein levels were upregulated in the miR‐541‐inhibiting BEAS‐2B cells. Lamin B₂ served as the internal control. A bigger field scan of the blot is shown in Fig. S1. (D) HMGA2 expression levels were enhanced in the nucleus of miR‐541‐inhibiting BEAS‐2B cells. (E) The RNA22 tool was used to predict that HMGA2 is a target gene of miR‐541. The predicted target site was on the 3′ UTR of HMGA2 mRNA. (F) Dual‐luciferase reporter assay analysis of the effect of miR‐541 expression on the 3′ UTR activity of HMGA2 mRNA in 293T cells (n = 3 experiments for all tests).