Figure 5.

Tunicamycin treatment affects GSK‐3 and CREB phosphorylation. After ventricular infusion of DMSO, TM at concentration of 50 μmol/L or TM plus SB (SB, 50 μmol/L) for 24 and 48 h, GSK‐3β levels of the hippocampal extract were measured by Western blotting (A) and quantitative analysis (B). SB treatment rescued the increased Y216‐GSK‐3β and decreased S9‐GSK‐3β induced by TM especially at 48 h. And for 48 h, SB treatment restored the levels of pSer133‐CREB and pSer129‐CREB measured by Western blotting (C) and quantitative analysis (D). The data were expressed as means ± SD (n = 6) * P < .05, ** P < .01 vs DMSO‐24 h, ^# P < .05 vs DMSO‐48 h, ^■ P < .05 vs TM‐24 h, ^▲ P < .05 vs TM‐48 h, ^& P < .05 vs TM + SB‐24 h in B. * P < .05 vs DMSO, ^# P < .05 vs TM in D