Figure 6.

Tunicamycin increases nuclear co‐localization of Y216‐GSK‐3β and pSer129‐CREB with reduced pSer133‐CREB. Rats were treated with TM or DMSO for 48 h and then the cytoplasmic and nuclear fractions of the hippocampus were analysed by Western blotting and quantitative analysis (A‐C). The representative immunofluorescence images show phosphorylated CREB probed by pSer133 and pSer129 (green), and phosphorylated GSK‐3β by Y216‐GSK‐3β (red) in cortex and hippocampus (bar = 50 μm) (D,E). SB treatment restored the nuclear internal and external metastasis of pSer133‐CREB and pSer129‐CREB. The co‐localization of pY216‐GSK‐3β with p‐S129‐CREB and p‐S133‐CREB in hippocampus were statistically measured. The data were expressed as means ± SD. The tendency of pY216‐GSK‐3β with p‐S129‐CREB and p‐S133‐CREB in cortex was similar that in hippocampus (F,G) and the statistical graph not be showed. (n = 6) * P < .05, ** P < .01, *** P < .001 vs DMSO in B and C. * P < .05 vs DMSO , ^# P < .05 vs TM in F and G