Figure 2.

Effect of GMP‐1 treatment on APP association with mitochondria in SH‐SY5Y neuroblastoma cells. A‐B, Coimmunoprecipitation of APP with anti‐Tom40 antibodies. SH‐SY5Y cells were treated with DMSO or 50 μmol/L of GMP‐1 for 16 h. A, Western blot results with anti‐APP (22c11) and anti‐Tom40 antibodies. B, Signal intensity calculated as ratio of APP/Tom40 antibody staining. DMSO‐treated sample was set as 100%. Experiments were performed in triplicates. C‐D, APP association with mitochondria and light membrane P3 fractions isolated from SH‐SY5Y cells treated with DMSO or 100 μmol/L of GMP‐1 for 16 h. C, Western blot results using anti‐APP (22c11) antibody. D, Signal intensity calculated as percentage of staining in mitochondrial or P3 samples to total sample staining. Three independent experiments were performed