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. 2018 Apr 17;22(7):3526–3536. doi: 10.1111/jcmm.13629

Figure 4.

Figure 4

miR‐1‐3p/miR‐206 reversed HGF‐induced gefitinib resistance by c‐Met. A, c‐Met knockdown mimicked the effect of miR‐1‐3p and miR‐206. Left, c‐Met knockdown was evaluated by western blot assay in PC‐9 and HCC827 cells. Right, PC‐9 and HCC827 cells with c‐Met knockdown were treated with or without gefitinib (1 μmol/L) in the presence/absence of HGF(50 ng/mL) for 72 h, cell viability was determined by MTT assay. Data are means of three separated experiments ± SD, **P < .01. B, Top, c‐Met overexpression was evaluated by Western blot assay in PC‐9 and HCC827 cells. Bottom, c‐Met overexpression attenuated the effects of miR‐1‐3p and miR‐206. PC‐9/HGF and HCC827/HGF cells were co‐transfected with c‐Met overexpression plasmid and miR‐1‐3p/miR‐206 mimics and then treated with gefitinib (1 μmol/L), cell viability was determined after 48 h of treatment by MTT assay. Data are means of three separated experiments ± SD, **P < .01