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. 2018 Apr 23;5(6):1701043. doi: 10.1002/advs.201701043

Figure 7.

Figure 7

a) Synthesis of gentamicin‐NONOate polymeric nanoparticles. Gentamicin is firstly conjugated to pre‐synthesized poly(oligoethylene glycol methyl ether methacrylate)‐b‐poly(3‐vinylbenzaldehyde) (POEGMA‐b‐PVBA) nanoparticles. Gentamicin has both primary and secondary amine groups, and in the next step, NO donor is directly formed by the reaction of gentamicin with NO gas to yield a unique gentamicin‐NONOate complex. b) Confocal microscope images of Pseudomonas aeruginosa biofilms stained with LIVE/DEAD kit. Biofilms are treated with NO donor spermine NONOate (Sper‐NO), free gentamicin, or gentamicin‐NONOate polymeric nanoparticles (Poly‐GEN‐NO). The gentamicin‐NONOate nanoparticles strongly kill bacterial biofilm compared to those inoculated with NO donors or gentamicin alone. Scale bar: 50 µm. Reproduced with permission.74 Copyright 2015, The Royal Society of Chemistry.