Figure 1.

The expression levels of CRB3 and β‐catenin correlate with TAM resistance of breast cancer. (A) Representative images of CRB3 and β‐catenin protein expression in primary tumour (PT) and metastatic tissues from patients who developed tamoxifen resistance and metastasis (TamR‐Met) (Table 1). (B) Representative images of CRB3 and β‐catenin IHC scores (0‐6) in PT and TamR‐Met. (C) CRB3 and β‐catenin are negatively correlated in TamR‐Met. (D) CRB3 and β‐catenin mRNA expression in human breast cancer cells evaluated by real‐time PCR. The expression levels of CRB3 mRNA in other cells were normalized to the one in LCC2 cells, and the expression levels of β‐catenin mRNA in other cells were normalized to the one in T47D cells. (E) Western blot analysis of CRB3 and β‐catenin expression in all cell lines. P values were obtained from Wilcoxon signed‐rank test (B), nonparametric Spearman's correlation (C) and unpaired t test (D)