Figure 7.

CRB3 affects cell properties through Wnt signalling in MCF7 cells. (A) The MCF7 cells were treated with various concentrations of tamoxifen. Cell viability was determined using an MTT assay. The percentage of cell viability was calculated from the OD values of the test groups normalized to the control groups. (B) FACS profiles and quantification of the CD44^high/CD24^low subpopulation. (C) Western blot analysis of β‐catenin, β‐catenin downstream factors CD44 and cMyc expressions. (D) CFSE analysis by FACS. P values were obtained from one‐way anova with Dunnett's multiple comparisons test (B)