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PDL‐1 is expressed in OS cell lines. Flow cytometry was performed using IgG‐APC or PDL‐1‐APC antibody. MFI (PDL‐1 positivity) normalized to IgG controls, standard deviations from three independent experiments are shown (A); Western blotting was performed using 10% SDS‐PAGE and anti‐hPDL‐1 antibody; MDA‐MB‐231 cells were positive control (B); ImageJ analysis was used for PDL‐1 (relative to actin) quantification (C).
