. 2018 May 2;8(11):5427–5440. doi: 10.1002/ece3.4065

Table 1.

Description of the six primers used for genotyping 778 individuals

Locus	Repeat	Primer sequence forward Primer sequence reverse	T _m (°C)	Product size	Range (bp)	GenBank accession no.	Mainland		Island		PHWE
Locus	Repeat	Primer sequence forward Primer sequence reverse	T _m (°C)	Product size	Range (bp)	GenBank accession no.	A	AR	A	AR
Locus 911	(CA)14	GCCTCGTCAAGAGTGGTCTC	54	259	236–269	KX641151	31	30.46	25	25	p < .001
Locus 911	(CA)14	GGAAAGCAGCAATTTTCTCG	54	259	236–269	KX641151	31	30.46	25	25	p < .001
Locus 1178	(TG)15	CACAGTACCCTGGAGGCATT	53	187	490–519	KX641152	25	24.641	28	28	p = .172
Locus 1178	(TG)15	CGTGAGAGAAATTTGCGTGA	53	187	490–519	KX641152	25	24.641	28	28	p = .172
Locus 1315	(TATAC)22	GCATGTGTGCAGTCTCGAAT	54	261	261–348	KX641153	14	13.956	15	14.994	p = .003
Locus 1315	(TATAC)22	GGGTCTGATGGAATACCGTG	54	261	261–348	KX641153	14	13.956	15	14.994	p = .003
Locus 434	(AC)28	AGCTCGGCTGATTCGTTATG	52	129	106–139	KX641154	22	21.885	19	19	p < .05
Locus 434	(AC)28	TTCTTTTCACTCGTGTTGCG	52	129	106–139	KX641154	22	21.885	19	19	p < .05
Locus 364	(CA)27	GCACTTTTCAGTGCCCATTT	53	330	91–144	KX641155					p < .001
Locus 364	(CA)27	CTGCTATTACTGGCACGCTG	53	330	91–144	KX641155					p < .001
Locus 592	(AC)18	CGAACGAATCCTTGGTCAAT	53	221	395–430	KX116456	19	18.852	18	17.994	p = .748
Locus 592	(AC)18	TACATCGTCCGACACAGCTC	53	221	395–430	KX116456	19	18.852	18	17.994	p = .748
Mean							22.2A	21.9588b	21A	20.9976b	p < .001

The variability at five microsatellite loci in Tapinoma melanocephalum was tested from mainland populations and island populations. The number of alleles (A) and allele richness (AR) were estimated among all samples. PHWE shows the p value for the test of Hardy–Weinberg equilibrium. Data with lowercase or uppercase letter indicate no significant difference between island populations and mainland populations using an independent t test (p > .05).