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. 2018 May 23;8(12):3366–3379. doi: 10.7150/thno.23978

Figure 5.

Figure 5

Functional relevance of rs372883 variants. (A) Relative reporter gene activity bearing BACH1 3'UTR fragment with the rs372883T or rs372883C allele in CFPAC-1 and BXPC-3 cells. Results are mean ± SEM from three experiments and each had six replicates. The P-values are for Student's t-test. (B) In silico prediction of interaction between miR-1257 and BACH1 3'UTR showing differences in binding within the seed region. (C) Relative reporter gene activity of the psiCHECK2-rs372883T and psiCHECK2-rs372883C constructs cotransfected with 1.0, 5.0 and 10.0 pmol of miR-1257 or its inhibitor in CFPAC-1 and BXPC-3 cells. Results are mean ± SEM from three experiments and each had six replicates; P-values were for Student's t-test. (D-F) Levels of BACH1 mRNA, miR-1257 and HMOX1 mRNA in normal pancreatic tissues adjacent to tumors of subjects with the rs372883 CC (N=11), CT (N=38) or TT (N=26) genotype. Results are mean ± SEM relative to GAPDH or U6. (G) Western blot analysis of AKT and ERK signaling modules downstream of HO-1 in surgically removed PDAC specimens from subjects with the rs372883CC, CT or TT genotype.