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. 2001 Oct 16;98(22):12659–12664. doi: 10.1073/pnas.171460798

Figure 1.

Figure 1

Analysis of the MCB-infiltrating B lymphoplasmacytic cells demonstrates an oligoclonal response. Amplification of the Ig heavy chain V(D)J segments from four MCB infiltrates (lanes 3, N-1; 4, N-21; 5, RH-12; and 6, RH-2) by reverse transcriptase-PCR and separation by gel electrophoresis resulted in three to five oligoclonal bands in the range of 80–130 bp for each of the tumors. In contrast, V(D)J amplification of a polyclonal B cell population obtained from peripheral blood lymphocytes of a healthy individual (lane 7) gave a smear pattern, whereas the V(D)J product of a human monoclonal antibody resulted in a single distinct band (lane 1). The negative control containing primers, but no DNA, is shown in lane 2. M, marker.