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. 2018 Jun 6;2018:7852742. doi: 10.1155/2018/7852742

Figure 2.

Figure 2

TaqMan Q-RT-PCR assays were performed using RNA samples from untreated macrophages or macrophages treated with LPS (100 ng/ml), NECA (1 μM), or LPS (100 ng/ml) with NECA (1 μM) for 12 hours. Samples from 3 separate experiments were each analyzed in duplicate, with data indicating mean fold change relative to untreated macrophages ± SE. Expression levels were normalized to the expression of cyclophilin-D as a housekeeping gene.