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. 2018 Jun 6;2018:7852742. doi: 10.1155/2018/7852742

Figure 5.

Figure 5

Analysis of the role of the miR-487b binding site in the 3′UTR of the Kat6b mRNA. The Kat6b 3′UTR was cloned downstream of the luciferase open reading frame in the pLightswitch-3′UTR reporter plasmid (pKat6bLuc-3′UTR). A second plasmid was prepared with the miR-487b binding site deleted (pKat6bLucΔ3′UTR). RAW264.7 macrophages were transfected with either pKat6bLuc-3′UTR or pKat6bLucΔ3′UTR. Transfected cells were then treated with LPS for 6 hr (100 ng/ml) and analyzed for luciferase expression (n = 3). RAW264.7 cells were also cotransfected with either pKat6bLuc-3′UTR or pKat6bLucΔ3′UTR together with either a synthetic miR-487b mimic or a nonspecific miR mimic (miR-433). The cells were then treated with LPS (100 ng/ml for 6 hr and analyzed for luciferase expression (n = 3). Data represent the mean ± SE. ∗ indicates samples with luciferase expression significantly different from control luciferase expression (p < 0.05).