Table 2.
Sequences of primers used in qPCR.
| Gene | Primer | Sequence 5′–3′ | Amplicon length (bp) | Accession no. |
|---|---|---|---|---|
| PPARγ | F: | TCCCTGTTTGTGTACAGCCTT | 191 | XM_014846252.1 |
| R: | CTCCATGGCTGATTTCCCCT | |||
|
| ||||
| STAT5A | F: | AGATGCTGGCCGAGGTCAAC | 212 | XM_001494738.4 |
| R: | AGACTTGGCCTGCTGCTCAC | |||
|
| ||||
| SREBP-1c | F: | TCAGCGAGGCGGCTTTGGAGCAG | 80 | XM_008542859.1 |
| R: | CATGTCTTCGATGTCGGTCAG | |||
|
| ||||
| p53 | F: | TTCCGCAAGAAGGAGGAACC | 114 | U37120.1 |
| R: | TTTGGACAGAACTGCACCCT | |||
|
| ||||
| p21 | F: | GAAGAGAAACCCCCAGCTCC | 241 | XM_014853747.1 |
| R: | TGACTGCATCAAACCCCACA | |||
|
| ||||
| BCL-2 | F: | TTCTTTGAGTTCGGTGGGGT | 164 | XM_014843802.1 |
| R: | GGGCCGTACAGTTCCACAA | |||
|
| ||||
| TET3 | F: | CAGCCTGCATGGACTTCTGT | 188 | XM_014731089.1 |
| R: | GTTCTCCTCACTGCCGAACT | |||
|
| ||||
| TET2 | F: | ATCCTGATCCTGGTGTGGGA | 143 | XM_005607932.2 |
| R: | CCTTGACAGGCACAGGTTCT | |||
|
| ||||
| DNMT1 | F: | GGCGAAAGCGGACAATTCTG | 90 | XM_014741825.1 |
| R: | AGCGGTCTAGCAACTGGTTC | |||
|
| ||||
| BECLIN-1 | F: | GATGCGTTATGCCCAGATGC | 233 | XM_014833759.1 |
| R: | AACGGCAGCTCCTCTGAAAT | |||
|
| ||||
| LC3 | F: | TTACTGCTTTGCTCTGCCAC | 213 | XM_014835085.1 |
| R: | AGCTGCTTCTCCCCCTTGTA | |||
|
| ||||
| LAMP2 | F: | GCACCCCTGGGAAGTTCTTA | 147 | XM_014831347.1 |
| R: | ATCCAGCGAACACTCTTGGG | |||
|
| ||||
| PINK | F: | GCACAATGAGCCAGGAGCTA | 298 | XM_014737247.1 |
| R: | GGGGTATTCACGCGAAGGTA | |||
|
| ||||
| PARKIN | F: | TCCCAGTGGAGGTCGATTCT | 218 | XM_014858374.1 |
| R: | CCCTCCAGGTGTGTTCGTTT | |||
|
| ||||
| MNF1 | F: | AAGTGGCATTTTTCGGCAGG | 217 | XM_001495170.5 |
| R: | TCCATATGAAGGGCATGGGC | |||
|
| ||||
| FIS1 | F: | GGTGCGAAGCAAGTACAACG | 118 | XM_001504462.4 |
| R: | GTTGCCCACAGCCAGATAGA | |||
|
| ||||
| EIF2α | F: | AGTCTTCAGGCATTGGCTCC | 489 | XM_001488848.5 |
| R: | CCGAGTGGGACATGTATCGG | |||
|
| ||||
| CHOP | F: | AGCCAAAATCAGAGCCGGAA | 272 | XM_001488999.3 |
| R: | GGGGTCAAGAGTGGTGAAGG | |||
|
| ||||
| PERK | F: | GTGACTGCAATGGACCAGGA | 283 | XM_014731045.1 |
| R: | TCACGTGCTCACGAGGATATT | |||
|
| ||||
| BIP | F: | CTGTAGCGTATGGTGCTGCT | 122 | XM_005606029.2 |
| R: | CATGACACCTCCCACGGTTT | |||
|
| ||||
| IL-6 | F: | CGTCACTCCAGTTGCCTTCT | 225 | XM_014830631.1 |
| R: | GCCAGTACCTCCTTGCTGTT | |||
|
| ||||
| GLUT4 | F: | TGGGCTCTCTCCGTGGCCATCTT | 658 | NM_001081866.2 |
| R: | GCTGCTGGCTGAGCTGCAGCA | |||
|
| ||||
| APLN | F: | TGGGCCTGAGATGGCTCTAT | 819 | XM_014729165.1 |
| R: | CCTCGGGGTTATTGTGGGAC | |||
|
| ||||
| GAPDH | F: | GATGCCCCAATGTTTGTGA | 250 | XM_014866500.1 |
| R: | AAGCAGGGATGATGTTCTGG | |||
Table 2 is reproduced from Kornicka et al. [10] (under the Creative Commons Attribution License/http://pubs.rsc.org/is/content/articlelanding/2016/ra/c6ra00029k), PPARγ—peroxisome proliferator-activated receptor gamma, STAT5A—signal transducer and activator of transcription 5A, SREBP-1c—sterol regulatory element binding transcription factor 1 (SREBF1), p53—tumor protein P53 (TP53), p21—cyclin dependent kinase inhibitor 1A (CDKN1A), BCL-2—B-cell lymphoma 2, TET3—Tet methylcytosine dioxygenase 3, TET2—Tet methylcytosine dioxygenase 2, DNMT1—DNA methyltransferase 1, BECLIN-1, LC3—microtubule-associated protein 1 light chain 3 beta (MAP1LC3B), LAMP2—lysosomal-associated membrane protein 2, PINK1—PTEN induced putative kinase 1, PARKIN—Parkin RBR E3 ubiquitin protein ligase (PRKN), MNF1—mitochondrial nucleoid factor 1, FIS1—fission, mitochondrial 1, EIF2α—eukaryotic translation initiation factor 2A, CHOP—DNA damage inducible transcript 3 (DDIT3), PERK—eukaryotic translation initiation factor 2-alpha kinase 3 (EIF2AK3), BIP—heat shock protein family A (Hsp70) member 5 (HSPA5), IL-6—interleukin 6, GLUT4—solute carrier family 2 member 4 (SLC2A4), APLN—apelin, GAPDH—glyceraldehyde 3-phosphate dehydrogenase.