Table 1.
Summary table of the results of the initial mutation test.
| Initial mutation test (plate incorporation test)∗ | ||||||||||||||||||||
|
| ||||||||||||||||||||
| Concentrations (μg/plate) | Salmonella typhimurium tester strains | Escherichia coli | ||||||||||||||||||
| TA 98 | TA 100 | TA 1535 | TA 1537 | WP2uvrA | ||||||||||||||||
| -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | |||||||||||
|
| ||||||||||||||||||||
| Mean values of revertants per plate and mutation rate (MR) | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR | Mean | MR |
|
| ||||||||||||||||||||
| Untreated control | 13.7 | 0.89 | 19.3 | 1.09 | 84.0 | 1.02 | 105.0 | 1.12 | 8.7 | 1.18 | 11.7 | 1.03 | 12.7 | 0.93 | 11.3 | 0.89 | 13.3 | 0.95 | 21.7 | 1.16 |
| DMSO control | 15.3 | 1.00 | 17.7 | 1.00 | 82.0 | 1.00 | 93.7 | 1.00 | 7.3 | 1.00 | 11.3 | 1.00 | 13.7 | 1.00 | 12.7 | 1.00 | 14.0 | 1.00 | 18.7 | 1.00 |
| Ultrapure Water control | – | – | – | – | 83.3 | 1.00 | – | – | 8.3 | 1.00 | – | – | – | – | – | – | 14.0 | 1.00 | – | – |
|
| ||||||||||||||||||||
| 5000 | 11.0 | 0.72 | 14.0 | 0.79 | 54.0 | 0.66 | 78.0 | 0.83 | 3.7 | 0.50 | 4.7 | 0.41 | 4.7 | 0.34 | 2.0 | 0.16 | 13.0 | 0.93 | 18.0 | 0.96 |
| 1600 | 13.3 | 0.87 | 18.0 | 1.02 | 56.0 | 0.68 | 70.3 | 0.75 | 9.3 | 1.27 | 8.0 | 0.71 | 12.7 | 0.93 | 9.7 | 0.76 | 10.7 | 0.76 | 18.0 | 0.96 |
| 500 | 12.3 | 0.80 | 22.0 | 1.25 | 60.7 | 0.74 | 86.3 | 0.92 | 8.7 | 1.18 | 10.0 | 0.88 | 10.0 | 0.73 | 12.0 | 0.95 | 11.3 | 0.81 | 19.3 | 1.04 |
| 160 | 11.0 | 0.72 | 23.0 | 1.30 | 89.3 | 1.09 | 92.3 | 0.99 | 8.0 | 1.09 | 11.3 | 1.00 | 11.7 | 0.85 | 12.7 | 1.00 | 14.3 | 1.02 | 19.7 | 1.05 |
| 50 | 15.3 | 1.00 | 24.0 | 1.36 | 98.3 | 1.20 | 87.0 | 0.93 | 7.3 | 1.00 | 9.7 | 0.85 | 12.0 | 0.88 | 13.7 | 1.08 | 11.0 | 0.79 | 16.7 | 0.89 |
| 16 | 15.3 | 1.00 | 20.0 | 1.13 | 86.0 | 1.05 | 91.7 | 0.98 | 9.0 | 1.23 | 9.0 | 0.79 | 12.0 | 0.88 | 12.7 | 1.00 | 14.0 | 1.00 | 17.7 | 0.95 |
| 5 | 13.3 | 0.87 | 17.0 | 0.96 | 84.3 | 1.03 | 101.0 | 1.08 | 9.0 | 1.23 | 12.0 | 1.06 | 13.3 | 0.98 | 12.3 | 0.97 | 14.0 | 1.00 | 18.3 | 0.98 |
|
| ||||||||||||||||||||
| NPD (4) | 208.0 | 13.57 | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SAZ (2) | – | – | – | – | 1008.0 | 12.10 | – | – | 1066.7 | 128.00 | – | – | – | – | – | – | – | – | – | – |
| 9AA (50) | – | – | – | – | – | – | – | – | – | – | – | – | 574.0 | 42.00 | – | – | – | – | – | – |
| MMS (2 μL) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 422.7 | 30.19 | – | – |
| 2AA (2) | – | – | 1469.3 | 83.17 | – | – | 2506.7 | 26.76 | – | – | 105.0 | 9.26 | – | – | 136.7 | 10.79 | – | – | – | – |
| 2AA (50) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | 209.0 | 11.20 |
∗DMSO was used as the vehicle for the test article and positive control substances: NPD, 9AA, and 2AA. Ultrapure water was used as the vehicle for SAZ and MMS. The mutation rate of the test item and the untreated control is given referring to the DMSO.